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Our Frozen Zoo® is the largest and most diverse collection of its kind in the world. It contains over 10,000 living cell cultures, oocytes, sperm, and embryos representing nearly 1,000 taxa, including one extinct species, the po’ouli. Located at the Beckman Center for Conservation Research, the collection is also duplicated for safekeeping at a second site. The irreplaceable living cell lines, gametes, and embryos stored in the Frozen Zoo® provide an invaluable resource for conservation, assisted reproduction, evolutionary biology, and wildlife medicine.
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During cell cycle, numerous proteins temporally and spatially localized in distinct sub-cellular regions including centrosome (spindle pole in budding yeast), kinetochore/centromere, cleavage furrow/midbody (related or homolog structures in plants and budding yeast called as phragmoplast and bud neck, respectively), telomere and spindle spatially and temporally. These sub-cellular regions play important roles in various biological processes. In this work, we have collected all proteins identified to be localized on kinetochore, centrosome, midbody, telomere and spindle from two fungi (S. cerevisiae and S. pombe) and five animals, including C. elegans, D. melanogaster, X. laevis, M. musculus and H. sapiens based on the rationale of "Seeing is believing" (Bloom K et al., 2005). Through ortholog searches, the proteins potentially localized at these sub-cellular regions were detected in 144 eukaryotes. Then the integrated and searchable database MiCroKiTS - Midbody, Centrosome, Kinetochore, Telomere and Spindle has been established.
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This database documents the expression and localization patterns of Drosophila mRNAs at the cellular and subcellular level during early embryogenesis and third instar larval tissues. A high-resolution, high-throughput fluorescence detection method is used to detect expressed mRNAs. The data can be accessed by searching the localization categories, searching for specific genes or browsing the list of tested genes.