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We developed a method, ChIP-sequencing (ChIP-seq), combining chromatin immunoprecipitation (ChIP) and massively parallel sequencing to identify mammalian DNA sequences bound by transcription factors in vivo. We used ChIP-seq to map STAT1 targets in interferon-gamma (IFN-gamma)-stimulated and unstimulated human HeLa S3 cells, and compared the method's performance to ChIP-PCR and to ChIP-chip for four chromosomes.For both Chromatin- immunoprecipation Transcription Factors and Histone modifications. Sequence files and the associated probability files are also provided.
The USGS Alaska Region has the largest geographic extent of the seven regional units within the USGS and represents a dynamic landscape of great natural wonder. It is a transforming landscape shaped by volcanoes, earthquakes, major rivers, and glaciers and a strategic landscape of yet untapped mineral and energy resources. The Region conducts research to help inform management of the extensive national parks and wildlife refuges of the far north and the international birds, fish, and marine mammals that migrate to these lands and waters; informs national Arctic energy policy through research on the National Petroleum Reserve-Alaska and the U.S. Outer Continental Shelf; and provides science to understand, help respond to and mitigate impacts from natural hazards. This work is accomplished in part by the Region's two Science Centers headquartered in Anchorage, the Alaska Science Center and the Volcano Science Center.
<<<!!!<<< This repository is no longer available. >>>!!!>>> NetPath is currently one of the largest open-source repository of human signaling pathways that is all set to become a community standard to meet the challenges in functional genomics and systems biology. Signaling networks are the key to deciphering many of the complex networks that govern the machinery inside the cell. Several signaling molecules play an important role in disease processes that are a direct result of their altered functioning and are now recognized as potential therapeutic targets. Understanding how to restore the proper functioning of these pathways that have become deregulated in disease, is needed for accelerating biomedical research. This resource is aimed at demystifying the biological pathways and highlights the key relationships and connections between them. Apart from this, pathways provide a way of reducing the dimensionality of high throughput data, by grouping thousands of genes, proteins and metabolites at functional level into just several hundreds of pathways for an experiment. Identifying the active pathways that differ between two conditions can have more explanatory power than just a simple list of differentially expressed genes and proteins.