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The taxonomically broad EST database TBestDB serves as a repository for EST data from a wide range of eukaryotes, many of which have previously not been thoroughly investigated. Most of the data contained in TBestDB has been generated by the labs of the Protist EST Program located in six universities across Canada. PEP is a large interdisciplinaryresearch project, involving six Canadian universities. PEP aims at the exploration of the diversity of eukaryotic genomes in a systematic, comprehensive and integrated way. The focus is on unicellular microbial eukaryotes, known as protists. Protistan eukaryotes comprise more than a dozen major lineages that, together, encompass more evolutionary, ecological and probably biochemical diversity than the multicellular kingdoms of animals, plants and fungi combined. PEP is a unique endeavor in that it is the first phylogenetically-broad genomic investigation of protists.