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The Brain Transcriptome Database (BrainTx) project aims to create an integrated platform to visualize and analyze our original transcriptome data and publicly accessible transcriptome data related to the genetics that underlie the development, function, and dysfunction stages and states of the brain.
We developed a method, ChIP-sequencing (ChIP-seq), combining chromatin immunoprecipitation (ChIP) and massively parallel sequencing to identify mammalian DNA sequences bound by transcription factors in vivo. We used ChIP-seq to map STAT1 targets in interferon-gamma (IFN-gamma)-stimulated and unstimulated human HeLa S3 cells, and compared the method's performance to ChIP-PCR and to ChIP-chip for four chromosomes.For both Chromatin- immunoprecipation Transcription Factors and Histone modifications. Sequence files and the associated probability files are also provided.
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Our Frozen Zoo® is the largest and most diverse collection of its kind in the world. It contains over 10,000 living cell cultures, oocytes, sperm, and embryos representing nearly 1,000 taxa, including one extinct species, the po’ouli. Located at the Beckman Center for Conservation Research, the collection is also duplicated for safekeeping at a second site. The irreplaceable living cell lines, gametes, and embryos stored in the Frozen Zoo® provide an invaluable resource for conservation, assisted reproduction, evolutionary biology, and wildlife medicine.
The Mouse Atlas of Gene Expression is a quantitative and comprehensive atlas of gene expression in mouse development. Gene expression levels from 198 tissue samples was measured using 202 Serial Analysis of Gene Expression (SAGE). Emphasis was on mouse development, samples taken at different stages of mouse development.