PhysioNet is an on-line forum for the dissemination and exchange of recorded biomedical signals and open-source software for analyzing them. It provides facilities for the cooperative analysis of data and the evaluation of proposed new algorithms. In addition to providing free electronic access to PhysioBank data and PhysioToolkit software via the World Wide Web. PhysioNet offers services and training via on-line tutorials to assist users with varying levels of expertise. PhysioNet is a resource for biomedical research and development. It has three closely interdependent components: PhysioBank is a large and growing archive of well-characterized digital recordings of physiologic signals, time series, and related data for use by the biomedical research community. PhysioBank currently includes more than 60 collections of cardiopulmonary, neural, and other biomedical signals from healthy subjects and patients with a variety of conditions with major public health implications, including sudden cardiac death, congestive heart failure, epilepsy, gait disorders, sleep apnea, and aging. PhysioToolkit is a large and growing library of software for physiologic signal processing and analysis, detection of physiologically significant events using both classical techniques and novel methods based on statistical physics and nonlinear dynamics, interactive display and characterization of signals, creation of new databases, simulation of physiologic and other signals, quantitative evaluation and comparison of analysis methods, and analysis of nonequilibrium and nonstationary processes. PhysioNetWorks is a virtual laboratory where you can work together with us and with colleagues anywhere in the world to create, evaluate, improve, document, and prepare new data and software "works" for publication on PhysioNet. Unlike all other parts of the PhysioNet web site, access to PhysioNetWorks is password-protected. (Accounts are free and a password can be obtained in a minute or two.)