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<<<!!!<<<This is an archive of the old NEBC site from nebc.nerc.ac.uk and is no longer updated. For new information regarding NERC Environmental Omics and the Bio-Linux system please see the EOS site at http://environmentalomics.org. >>>!!!>>> Ongoing NEBC activities, including the development of Bio-Linux, are being moved into the new EOS programme http://environmentalomics.org/portfolio/big-data-infrastructure/ . Once the current material from this website has been moved into EOS, this NEBC site will remain on-line as an archive. EnvBase is the searchable index to the data deposited through the NEBC, as well as related NERC experimental data. At present this is chiefly from the grants funded by the NERC Environmental Genomics Science Programme and the subsequent Post-genomics and Proteomics Science Programme, but more data from ongoing projects continues to be added
The EZRC at KIT houses the largest experimental fish facility in Europe with a capacity of more than 300,000 fish. Zebrafish stocks are maintained mostly as frozen sperm. Frequently requested lines are also kept alive as well as a selection of wildtype strains. Several thousand mutations in protein coding genes generated by TILLING in the Stemple lab of the Sanger Centre, Hinxton, UK and lines generated by ENU mutagenesis by the Nüsslein-Volhard lab in addition to transgenic lines and mutants generated by KIT groups or brought in through collaborations. We also accept submissions on an individual basis and ship fish upon request to PIs in Europe and elsewhere. EZRC also provides screening services and technologies such as imaging and high-throughput sequencing. Key areas include automation of embryo handling and automated image acquisition and processing. Our platform also involves the development of novel microscopy techniques (e.g. SPIM, DSLM, robotic macroscope) to permit high-resolution, real-time imaging in 4D. By association with the ComPlat platform, we can support also chemical screens and offer libraries with up to 20,000 compounds in total for external users. As another service to the community the EZRC provides plasmids (cDNAs, transgenes, Talen, Crispr/cas9) maintained by the Helmholtz repository of Bioparts (HERBI) to the scientific community. In addition the fish facility keeps a range of medaka stocks, maintained by the Loosli group.